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integrin β2 yfp plasmid  (Thermo Fisher)


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    Structured Review

    Thermo Fisher integrin β2 yfp plasmid
    Dissociation constant (K d ) values determined for <t> integrin </t> <t> β2 </t> tail and Dok1 interactions <xref ref-type= *. " width="250" height="auto" />
    Integrin β2 Yfp Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/integrin+%CE%B22+yfp+plasmid/pmc04479986-136-14-36
    Average 86 stars, based on 1 article reviews
    integrin β2 yfp plasmid - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding"

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding

    Journal: Scientific Reports

    doi: 10.1038/srep11630

    Dissociation constant (K d ) values determined for integrin β2 tail and Dok1 interactions <xref ref-type= *. " title="Dissociation constant (K d ) values determined for integrin β2 tail and Dok1 ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Dissociation constant (K d ) values determined for integrin β2 tail and Dok1 interactions *.

    Techniques Used:

    ( A ) K562 cells transfected with the indicated expression plasmids were lysed. Proteins were resolved on 10% SDS-PAGE under reducing conditions and immunoblotted (IB) with either anti-GFP or anti-Dok1 antibodies. ( B ) Transfected K562 cells were subjected to YFP-photobleach FRET analyses. Each data point represents the mean ± S.D. of ≥30 cells analyzed. ( C ) Flow cytometry analyses of K562 stable line expressing Dok1-CFP that were transfected with integrin αLβ2-YFP and CCR5. Wild-type K562 cells were used as the control group. ( D ) FRET analyses of cells in ( C ) that were treated without or with chemokine RANTES (50 ng/ml) for 10 min at 37 °C. 60 and 39 cells were analyzed for conditions without and with RANTES treatment, respectively. Data point represent mean ± S.D. *,p < 0.05, Student’s t test.
    Figure Legend Snippet: ( A ) K562 cells transfected with the indicated expression plasmids were lysed. Proteins were resolved on 10% SDS-PAGE under reducing conditions and immunoblotted (IB) with either anti-GFP or anti-Dok1 antibodies. ( B ) Transfected K562 cells were subjected to YFP-photobleach FRET analyses. Each data point represents the mean ± S.D. of ≥30 cells analyzed. ( C ) Flow cytometry analyses of K562 stable line expressing Dok1-CFP that were transfected with integrin αLβ2-YFP and CCR5. Wild-type K562 cells were used as the control group. ( D ) FRET analyses of cells in ( C ) that were treated without or with chemokine RANTES (50 ng/ml) for 10 min at 37 °C. 60 and 39 cells were analyzed for conditions without and with RANTES treatment, respectively. Data point represent mean ± S.D. *,p < 0.05, Student’s t test.

    Techniques Used: Transfection, Expressing, SDS Page, Flow Cytometry

    Comparison of amino acid sequences of integrin cytoplasmic tails highlighting the NxxY/F motif (blue) and potential phosphorylation of Ser residues (red).
    Figure Legend Snippet: Comparison of amino acid sequences of integrin cytoplasmic tails highlighting the NxxY/F motif (blue) and potential phosphorylation of Ser residues (red).

    Techniques Used:

    Related Articles

    Transfection:

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding
    Article Snippet: K562 cells (ATCC, Manassas, VA) were cultured in RPMI1640 full-medium containing 10% (v/v) FBS and antibiotics. .. Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen). ..

    Plasmid Preparation:

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding
    Article Snippet: K562 cells (ATCC, Manassas, VA) were cultured in RPMI1640 full-medium containing 10% (v/v) FBS and antibiotics. .. Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen). ..

    Mutagenesis:

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding
    Article Snippet: K562 cells (ATCC, Manassas, VA) were cultured in RPMI1640 full-medium containing 10% (v/v) FBS and antibiotics. .. Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen). ..

    Electroporation:

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding
    Article Snippet: K562 cells (ATCC, Manassas, VA) were cultured in RPMI1640 full-medium containing 10% (v/v) FBS and antibiotics. .. Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen). ..

    Transferring:

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding
    Article Snippet: K562 cells (ATCC, Manassas, VA) were cultured in RPMI1640 full-medium containing 10% (v/v) FBS and antibiotics. .. Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen). ..



    Similar Products

    integrin β2 yfp plasmid  (Thermo Fisher)
    86
    Thermo Fisher integrin β2 yfp plasmid
    Dissociation constant (K d ) values determined for <t> integrin </t> <t> β2 </t> tail and Dok1 interactions <xref ref-type= *. " width="250" height="auto" />
    Integrin β2 Yfp Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/integrin+%CE%B22+yfp+plasmid/pmc04479986-136-14-36
    Average 86 stars, based on 1 article reviews
    integrin β2 yfp plasmid - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Dissociation constant (K d ) values determined for integrin β2 tail and Dok1 interactions <xref ref-type= *. " width="100%" height="100%">

    Journal: Scientific Reports

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding

    doi: 10.1038/srep11630

    Figure Lengend Snippet: Dissociation constant (K d ) values determined for integrin β2 tail and Dok1 interactions *.

    Article Snippet: Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen).

    Techniques:

    ( A ) K562 cells transfected with the indicated expression plasmids were lysed. Proteins were resolved on 10% SDS-PAGE under reducing conditions and immunoblotted (IB) with either anti-GFP or anti-Dok1 antibodies. ( B ) Transfected K562 cells were subjected to YFP-photobleach FRET analyses. Each data point represents the mean ± S.D. of ≥30 cells analyzed. ( C ) Flow cytometry analyses of K562 stable line expressing Dok1-CFP that were transfected with integrin αLβ2-YFP and CCR5. Wild-type K562 cells were used as the control group. ( D ) FRET analyses of cells in ( C ) that were treated without or with chemokine RANTES (50 ng/ml) for 10 min at 37 °C. 60 and 39 cells were analyzed for conditions without and with RANTES treatment, respectively. Data point represent mean ± S.D. *,p < 0.05, Student’s t test.

    Journal: Scientific Reports

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding

    doi: 10.1038/srep11630

    Figure Lengend Snippet: ( A ) K562 cells transfected with the indicated expression plasmids were lysed. Proteins were resolved on 10% SDS-PAGE under reducing conditions and immunoblotted (IB) with either anti-GFP or anti-Dok1 antibodies. ( B ) Transfected K562 cells were subjected to YFP-photobleach FRET analyses. Each data point represents the mean ± S.D. of ≥30 cells analyzed. ( C ) Flow cytometry analyses of K562 stable line expressing Dok1-CFP that were transfected with integrin αLβ2-YFP and CCR5. Wild-type K562 cells were used as the control group. ( D ) FRET analyses of cells in ( C ) that were treated without or with chemokine RANTES (50 ng/ml) for 10 min at 37 °C. 60 and 39 cells were analyzed for conditions without and with RANTES treatment, respectively. Data point represent mean ± S.D. *,p < 0.05, Student’s t test.

    Article Snippet: Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen).

    Techniques: Transfection, Expressing, SDS Page, Flow Cytometry

    Comparison of amino acid sequences of integrin cytoplasmic tails highlighting the NxxY/F motif (blue) and potential phosphorylation of Ser residues (red).

    Journal: Scientific Reports

    Article Title: An Alternative Phosphorylation Switch in Integrin β2 (CD18) Tail for Dok1 Binding

    doi: 10.1038/srep11630

    Figure Lengend Snippet: Comparison of amino acid sequences of integrin cytoplasmic tails highlighting the NxxY/F motif (blue) and potential phosphorylation of Ser residues (red).

    Article Snippet: Cells (2 × 10 6 ) were transfected with integrin αL plasmid (9 μg), integrin β2-YFP plasmid (WT or mutant) (9 μg) and Dok1-CFP or CFP plasmid (12 μg) by electroporation using the Neon electroporation kit (Invitrogen) on a MP-100 pipette-type microporator (Invitrogen).

    Techniques: